A comparison study of GeneXpert and In-House N1N2 CDC Real-Time RT-PCR for detection of SARS-CoV-2 infection

COVID-19 is a disease caused by SARS-CoV-2, new virus from genus β-coronaviruses. This has been declared pandemic WHO on 11 March 2020 until now. The nucleic acid tests are the most frequently used assays because of their high sensitivity and specificity. One GeneXpert, real-time reverse transcription polymerase chain reaction (rRT-PCR)-based assay platform. use GeneXpert shows great public health interest rapid (50 min), minimum number trained staff, less infrastructure equipment. However, there limited data application for detection SARS-CoV-2. Therefore, we conducted comparative study between in-house N1N2 CDC rRT-PCR assay. Of 86 samples, 17 were positive while 13 positive. 7 negative [58.82% (10/17] sensitivity]. We also found that 3 samples showed (95.65% [66/69] specificity). It concluded results can not rule out possibility SARS-CoV-2 infection, particularly in close-contact individuals interpretation result should be analyzed carefully, amplification with Ct>40.